Monsanto issued US patent for rice transgenic event 17314 and methods of use

ALEXANDRIA, Virginia , January 9, 2014 () – According to news reporting originating from Alexandria, Virginia, by NewsRx journalists, a patent by the inventors Chen, Yun-Chia Sophia (Milpitas, CA); Duong, Can (St. Louis, MO); Hoi, Sio-Wai (St. Louis, MO); Hubmeier, Christopher S. (Ballwin, MO); Qi, Youlin (Chesterfield, MO), filed on March 29, 2010, was published online on December 31, 2013 (see also Biotechnology Companies).

The assignee for this patent, patent number 8618360, is Monsanto Technology LLC (St. Louis, MO).

Reporters obtained the following quote from the background information supplied by the inventors: "Rice is an important crop in many areas of the world. The methods of biotechnology have been applied to rice in order to produce rice with improved traits. One such improved trait is herbicide tolerance. The expression of an herbicide tolerance transgene in a plant is useful for the purpose of producing a plant having the desirable characteristic of herbicide tolerance. The expression of a transgene in a plant may be influenced by the chromosomal location of the transgene, perhaps due to chromatin structure (e.g., heterochromatin), or the proximity of transcriptional regulatory elements (e.g., enhancers) close to the integration site. For this reason, it is often necessary to screen a large number of individual plant transformation events in order to identify an event having optimal expression of a transgene and therefore the specific desirable characteristic. For example, it has been observed in plants that there may be wide variation in the level of transgene expression among events. There may also be differences in spatial or temporal patterns of expression, e.g., differences in the relative transgene expression levels in various plant tissues, that may not correspond to the patterns expected from transcriptional regulatory elements present in the introduced gene construct. For this reason, it may be necessary to produce several hundred to several thousand different transgenic events and screen these for an event that has the desired transgene expression levels and patterns for commercial purposes. Such an event having the desired levels or patterns of transgene expression may then be used for introgressing the transgene into other genetic backgrounds by sexual crossing using plant breeding methods. Progeny of such crosses would have the transgene expression characteristics of the original transformant. This may be used to ensure reliable gene expression in a number of different varieties that are suitably adapted to specific local growing conditions."

In addition to obtaining background information on this patent, NewsRx editors also obtained the inventors' summary information for this patent: "The present invention provides transgenic rice plants comprising event 17314, which exhibit commercially acceptable tolerance to applications of glyphosate herbicide, having representative seed deposited with American Type Culture Collection (ATCC) with Accession No. PTA-9844. The invention also provides seeds, progeny, plant parts, cells, and commodity products of rice comprising event 17314. The invention also provides novel DNA molecules related to the genome of rice comprising event 17314 and methods of using these molecules. The invention also provides methods of using transgenic rice event 17314 and plants comprising the event and methods of producing glyphosate tolerant rice.

"The present invention provides DNA molecules related to rice event 17314. These DNA molecules may comprise nucleotide sequences representing or derived from: the junction between the transgene insertion and flanking genomic DNA of rice event 17314, and/or a region of the genomic DNA flanking the inserted DNA, and/or a region of the integrated transgenic DNA flanking the insertion site, and/or a region of the integrated transgenic expression cassette, and/or a contiguous sequence of any of these regions. The present invention also provides DNA molecules useful as primers and probes diagnostic for rice event 17314. Rice plants, plant cells, plant parts, commodity products, progeny, and seeds comprising these molecules are also disclosed.

"The present invention provides methods, compositions, and kits useful for detecting the presence of DNA derived from rice event 17314. The present invention provides a method for detection of event 17314 by contacting a sample comprising DNA with a primer set that when used in a nucleic acid amplification reaction with genomic DNA from rice event 17314 produces an amplified DNA diagnostic for rice event 17314, performing a nucleic acid amplification reaction thereby producing the amplified DNA, and detecting the amplified DNA. The present invention also provides a method for detection of event 17314 by contacting a sample comprising DNA with a probe that when used in a hybridization reaction with genomic DNA from event 17314 hybridizes to a DNA molecule specific for rice event 17314, performing a hybridization reaction, and detecting the hybridization of the probe to the DNA molecule. Kits comprising the methods and compositions of the present invention useful for detecting the presence of DNA derived from rice event 17314 are also provided.

"The present invention provides a rice plant, seed, plant cell, progeny plant, plant part, or commodity product derived from a plant, plant cell, or seed comprising event 17314. The present invention also provides a rice plant, seed, plant cell, progeny plant, plant part, or commodity product comprising a DNA molecule having a nucleotide sequence selected from the group consisting of SEQ ID NO: 1-6, and complements and fragments thereof or a DNA molecule comprising at least 90% sequence identity to SEQ ID NO:6. The present invention also provides a rice plant, seed, plant cell, progeny plant, plant part, or commodity product derived from the plant or seed comprising event 17314 or comprising a DNA molecule that produces an amplified DNA molecule comprising SEQ ID NO: 1 and/or SEQ ID NO: 2, for instance in a DNA amplification method.

"The present invention provides a method for controlling weeds in a field by planting rice comprising event 17314 and then applying an effective dose of glyphosate herbicide capable of controlling the weeds without injuring the plants comprising event 17314.

"The present invention provides methods of producing a rice plant and/or seed that tolerates application of glyphosate herbicide by sexually crossing a rice plant comprising event 17314 or comprising SEQ ID NO: 1 or SEQ ID NO: 2 with a second rice plant, thereby producing seed, growing the seed to produce progeny plants, treating the progeny plants with glyphosate, and selecting a progeny plant that is tolerant to glyphosate. The methods may also include selfing the selected progeny plant to produce a plurality of second generation progeny plants and selecting from these a glyphosate tolerant plant. The methods may also include sexually crossing the selected progeny plant with another rice plant to produce seed, growing the seed to produce a second generation of progeny plants, treating the second generation of progeny plants with glyphosate, and selecting a second generation progeny plant that is tolerant to glyphosate. The present invention provides methods of producing a rice plant and/or seed that tolerates application of glyphosate herbicide by selfing a glyphosate tolerant plant comprising event 17314 and comprising SEQ ID NO: 1 or SEQ ID NO: 2, thereby producing seed, growing the seed to produce progeny plants, treating the progeny plants with glyphosate; and selecting a progeny plant that is tolerant to glyphosate.

"The foregoing and other aspects of the invention will become more apparent from the following detailed description."

For more information, see this patent: Chen, Yun-Chia Sophia; Duong, Can; Hoi, Sio-Wai; Hubmeier, Christopher S.; Qi, Youlin. Rice Transgenic Event 17314 and Methods of Use. U.S. Patent Number 8618360, filed March 29, 2010, and published online on December 31, 2013. Patent URL: http://patft.uspto.gov/netacgi/nph-Parser?Sect1=PTO2&Sect2=HITOFF&p=74&u=%2Fnetahtml%2FPTO%2Fsearch-bool.html&r=3653&f=G&l=50&co1=AND&d=PTXT&s1=20131231.PD.&OS=ISD/20131231&RS=ISD/20131231

Keywords for this news article include: Herbicides, Pesticides, DNA Research, Monsanto Company, Biotechnology Companies, Monsanto Technology LLC.

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